plasmids serpine1  (Genechem)

Journal: Cancer cell international

Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.

doi: 10.1186/s12935-025-03868-x

Figure Lengend Snippet: Fig. 1 Schematic representation of the process used to identify an oncogene regulated by H19 based on total RNA-seq data (GSE243116). SERPINE1 was identified as a potential oncogene upregulated by H19 in GBM cell lines (p < 0.05).

Article Snippet: A pcDNA3.1 vector containing a fulllength H19 construct (pcDNA3.1-H19) and a pCMVSPORT6 vector containing a full-length SERPINE1 construct (pCMV-SPORT6-SERPINE1) were purchased from Addgene (Cambridge, MA, USA) and the Korea Human Gene Bank (Medical Genomics Research Center, KRIBB, Daejeon, Republic of Korea), respectively.

Techniques: RNA Sequencing

Journal: Cancer cell international

Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.

doi: 10.1186/s12935-025-03868-x

Figure Lengend Snippet: Fig. 3 Identification of miR-19b-3p as a tumor suppressor that binds to H19 and SERPINE1 in GBM cell lines. (A) miRNAs that bind to H19 and SERPINE1 were predicted using RNA interactome databases. (B) Overall survival analysis of miR-19b-3p (p = 0.004) in GBM patients was performed using the On coLnc database. (C) The miR-19b-3p sequence responsible for binding to H19 (H19-WT) and the mutant sequence of H19 (H19-MUT) were predicted using the ENCORI database. (D) The binding sequence responsible for miR-19b-3p binding to SERPINE1 (SERPINE1-WT) and the mutant sequence of SERPINE1 (SERPINE1-MUT) were also predicted using the ENCORI database. (E) The binding activities of miR-19b-3p mimic with H19-WT or H19-MUT were evaluated using a dual-luciferase reporter assay. (F) The binding activities of miR-19b-3p mimic with SERPINE1-WT or SERPINE1-MUT were also evaluated using a dual-luciferase reporter assay. **p < 0.01, ***p < 0.001

Article Snippet: A pcDNA3.1 vector containing a fulllength H19 construct (pcDNA3.1-H19) and a pCMVSPORT6 vector containing a full-length SERPINE1 construct (pCMV-SPORT6-SERPINE1) were purchased from Addgene (Cambridge, MA, USA) and the Korea Human Gene Bank (Medical Genomics Research Center, KRIBB, Daejeon, Republic of Korea), respectively.

Techniques: Sequencing, Binding Assay, Mutagenesis, Luciferase, Reporter Assay

Journal: Cancer cell international

Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.

doi: 10.1186/s12935-025-03868-x

Figure Lengend Snippet: Fig. 6 A schematic diagram of the ceRNA network illustrating the interaction between H19, miR-19b-3p, and SERPINE1. In this network, H19 competes with SERPINE1 mRNA for binding to miR-19b-3p. When H19 acts as a ceRNA sponging miR-19b-3p, the binding of miR-19b-3p to SERPINE1 mRNA is reduced. This reduction alleviates the inhibition of SERPINE1 translation by miR-19b-3p, ultimately leading to the upregulation of SERPINE1 in response to increased H19 expression

Article Snippet: A pcDNA3.1 vector containing a fulllength H19 construct (pcDNA3.1-H19) and a pCMVSPORT6 vector containing a full-length SERPINE1 construct (pCMV-SPORT6-SERPINE1) were purchased from Addgene (Cambridge, MA, USA) and the Korea Human Gene Bank (Medical Genomics Research Center, KRIBB, Daejeon, Republic of Korea), respectively.

Techniques: Binding Assay, Inhibition, Expressing